Help with Genetic Cloning, Splicing, and Sequencing Practice Test
•2 QuestionsTwo students receive 1 microliter of plasmid DNA that expressed beta-lactamase under a constitutive promoter, and green fluorescent protein (eGFP) under an arabinose inducible promoter.
The students decide to heat shock transform the entire volume of plasmid DNA into chemically competent bacterial cells to ensure that they have enough replicated plasmid for future experiments. The students also transform the same volume of sterile water as a control. They plate their transformations on the following plates:
1. Nutrient agar
2. Nutrient agar plus ampicillin
3. Nutrient agar plus ampicillin and arabinose
The next day, the students observe a lawn of bacteria on each plate for bacterica transformed with the plasmid, with only 1/10 of the bacteria glowing green on plate 3. Additionally, they observe a lawn of bacteria on each plate for the control condition.
Which is most likely the cause of their results?
Two students receive 1 microliter of plasmid DNA that expressed beta-lactamase under a constitutive promoter, and green fluorescent protein (eGFP) under an arabinose inducible promoter.
The students decide to heat shock transform the entire volume of plasmid DNA into chemically competent bacterial cells to ensure that they have enough replicated plasmid for future experiments. The students also transform the same volume of sterile water as a control. They plate their transformations on the following plates:
1. Nutrient agar
2. Nutrient agar plus ampicillin
3. Nutrient agar plus ampicillin and arabinose
The next day, the students observe a lawn of bacteria on each plate for bacterica transformed with the plasmid, with only 1/10 of the bacteria glowing green on plate 3. Additionally, they observe a lawn of bacteria on each plate for the control condition.
Which is most likely the cause of their results?