Understanding Cloning and PCR Practice Test

1. Use a restriction enzyme to digest HBB

2. Amplify the expression vector via PCR

3. Ligate the digested HBB and vector

1. Use a restriction enzyme to digest the expression vector

2. Ligate HBB and the digested vector

3. Amplify HBB via PCR

1. Ligate HBB

2. Use a restriction enzyme to digest the expression vector

3. Amplify HBB and the digested expression vector via PCR

1. Amplify HBB via PCR

2. Use a restriction enzyme to digest the expression vector

3. Ligate HBB and the digested vector

None of the other answers