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Bioenergetics and Biological Oxidation–Reduction (5E) Practice Test
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Q1
Isolated mitochondria were supplied with malate to drive NADH production in the matrix and oxygen as the terminal electron acceptor. NADH oxidation was monitored while ADP (1.0 mM) and inorganic phosphate (Pi, 10 mM) were present. The experiment was repeated after adding rotenone, an inhibitor that prevents electron transfer from NADH dehydrogenase (Complex I) to ubiquinone (Q). Under these conditions, ATP synthase remains functional and the inner membrane is intact.
Which prediction would be expected given the redox reaction?
Isolated mitochondria were supplied with malate to drive NADH production in the matrix and oxygen as the terminal electron acceptor. NADH oxidation was monitored while ADP (1.0 mM) and inorganic phosphate (Pi, 10 mM) were present. The experiment was repeated after adding rotenone, an inhibitor that prevents electron transfer from NADH dehydrogenase (Complex I) to ubiquinone (Q). Under these conditions, ATP synthase remains functional and the inner membrane is intact.
Which prediction would be expected given the redox reaction?