Physical Chemistry : Michaelis-Menton Analysis
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Example Questions
Example Question #1 : Michaelis Menton Analysis
Which of the following is true regarding the Michaelis constant?
It is the enzyme concentration at which the reaction rate is at maximum
It is the enzyme concentration at which the reaction rate is half of maximum
It is the substrate concentration at which the reaction rate is at maximum
It is the substrate concentration at which the reaction rate is half of maximum
It is the substrate concentration at which the reaction rate is half of maximum
Michaelis constant, or 
Example Question #2 : Equilibrium And Kinetics
Which of the following will have the greatest increase in reaction rate?
Increasing the Michaelis constant by a factor of 2
Decreasing the substrate concentration by a factor of 2
Increasing the maximum velocity by a factor of 2
Increasing the substrate concentration by a factor of 2
Increasing the maximum velocity by a factor of 2
Reaction rate, according to Michaelis-Menten model is as follows.
where 
Note that the units for 
Example Question #2 : Michaelis Menton Analysis
Consider the following reaction parameters.
Substrate concentration =
Michaelis constant =
What can you conclude about the reaction rate?
The reaction rate is
The reaction rate is
The reaction rate is
The reaction rate cannot be determined from the given information
The reaction rate is
To solve this problem we need to use the Michaelis-Menten equation.
where 
Note that the Michaelis-Menten equation implies that the 
Example Question #3 : Michaelis Menton Analysis
The Michaelis-Menten model implies that __________ the Michaelis constant will __________ the reaction rate.
decreasing . . . not change
decreasing . . . decrease
increasing . . . decrease
increasing . . . not change
increasing . . . decrease
The Michaelis-Menten equation is as follows.
Where 
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