All GRE Subject Test: Biology Resources
Example Questions
Example Question #1 : Microbiology
Which of the following is the best description of a bacteriophage?
A living organism
An obligate intracellular parasite
A prokaryote
A non-living organism
A fungus
An obligate intracellular parasite
A bacteriophage is a virus that infects bacteria. They are not considered living (becuase they cannot replicate on their own) organisms, nor are they techincally considered non-living organisms. They are called obligate intracellular organisms, because they are parasites (kill the cell) that require a host in order to replicate.
Example Question #1 : Understanding Bacteriophages
A lytic bacteriophage produces "substance X," which causes a host cell to lyse. At which point would the gene for substance X most likely be expressed?
Prior to viral infection
Immediately after viral infection
After all viruses are released
After integration into the host chromosome
Late after viral infection
Late after viral infection
Substance X would logically be released in the later part of viral infection. This would be referred to as a "late gene." The reason is that the virus needs time to replicate and assemble its progeny in the host. Lysing the cell immediately after infection would be futile since no progeny could be created. Lytic viruses use the lysis event to escape from the cell, so it cannot occur after viral release. Finally, lytic viruses typically do not integrate into the host chromosome, lysogenic viruses do.
Example Question #2 : Understanding Bacteriophages
What allows bacteriophages to only infect bacteria cells?
They are retroviruses
They harbor mosaic genomes due to site-specific recombination or illegitimate recombination
They attach to lipopolysaccharide and teichoic acid surface receptors
They carry out the lytic cycle
They carry out the lysogenic cycle
They attach to lipopolysaccharide and teichoic acid surface receptors
The cell surface receptors that a virus recognizes and binds provides specificity for the organism(s) that it infects. Both lipopolysaccharides and teichoic acids are cell surface proteins of bacteria. Bacteriophages have very diverse mosaic genomes and replicate through both the lytic and lysogenic cycles. Retroviruses are RNA-positive strand viruses, which uses reverse transcriptase to synthesize DNA from an RNA template. Its DNA form of its genome is integrated into the host cell's genome.
Example Question #1 : Viruses
Which of the following statements describes a lysogenic infection?
The virus enters the cell, hijacks host reproductive machinery, and creates new viruses that will destroy the cell
A lysogenic infection can only be done by DNA viruses
The virus incorporates its nucleic acid into the host's genome
The virus will inject its genome into the host cell through its tail
The virus incorporates its nucleic acid into the host's genome
A lysogenic infection involves the virus entering the cell and incorporating its nucleic acid into the host genome. RNA viruses are capable of this type of infection, but must first have their RNA be converted to DNA using the enzyme reverse transcriptase. The DNA genome remains dormant in the cell as it undergoes cellular divisions, increasing the number of cells that contain the viral DNA. This stage is known as the latent period.
Eventually, the viral genome will be stimulated and enter into the lytic viral cycle. During this phase, the virus hijacks the cell ribosomes and produces large quantities of the viral genome and proteins. Eventually the virus causes the cell to lyse, releasing more virions into the host.
Example Question #1 : Microbiology
Which of the following is not a component of HIV?
RNA
Ribosomes
Proteases
Reverse transcriptase
Protein
Ribosomes
HIV is a retrovirus, meaning that it contains RNA as its genetic material, and thus reverse transcriptase to code for DNA from the RNA template. It also has a protein coat, and must use proteases in order to degrade the host cell membrane to inject its RNA. It does not, however, have ribosomes. It will eventually use the host ribosomes to translate its own genetic material.
Example Question #2 : Microbiology
Which of the following is not a described type of virus?
I. A virus containing double strand DNA
II. A virus containing single strand DNA
III. A virus containing single strand RNA and single strand DNA
IV. A virus containing single strand RNA
V. A virus containing double strand RNA
III
IV
II
I
V
III
The question addresses the Baltimore classification system for viral genetic information. Viruses contain some form of DNA or RNA, but never both.
Example Question #1 : Microbiology
What molecule would you not expect to find in a retrovirus?
Uracil
Cytosine
Thymine
Adenine
Guanine
Thymine
Retroviruses carry RNA and when they infect a host cell, they use their own reverse transciptase to made DNA from that RNA; therefore, within the virus, there are only the molecules that make up RNA. Uracil is used in RNA place of thymine, which can be found in DNA. Cytosine, guanine, and adenine can be found in both DNA and RNA.
Example Question #4 : Divisions Of Life And Viruses
Sexually transmitted diseases are a common problem among young people in the United States. One of the more common diseases is caused by the bacterium Neisseria gonorrhoeae, which leads to inflammation and purulent discharge in the male and female reproductive tracts.
The bacterium has a number of systems to evade host defenses. Upon infection, it uses pili to adhere to host epithelium. The bacterium also uses an enzyme, gonococcal sialyltransferase, to transfer a sialyic acid residue to a gonococcal surface lipooligosaccharide (LOS). A depiction of this can be seen in Figure 1. The sialyic acid residue mimics the protective capsule found on other bacterial species.
Once infection is established, Neisseria preferentially infects columnar epithelial cells in the female reproductive tract, and leads to a loss of cilia on these cells. Damage to the reproductive tract can result in pelvic inflammatory disease, which can complicate pregnancies later in the life of the woman.
Which of the following is true of Neisseria, but not true of a virus?
Neisseria contains DNA; all viruses contain RNA
Neisseria is enveloped; no viruses are enveloped
Neisseria infects only one cell type; viruses have wide variety in their cellular targets
Neisseria has ribosomes; viruses do not have ribosomes
Neisseria does not have flagella; all viruses have flagella
Neisseria has ribosomes; viruses do not have ribosomes
Neisseria synthesizes proteins as a free living prokaryote. Viruses are generally dependent on host cellular machinery, and synthesize their proteins upon hijacking of a host ribosome.
Example Question #5 : Viruses
What component is common for all viruses?
DNA
RNA
A protective envelope
A protein coat
A protein coat
All viruses contain a protein coat (or capsule) that protects the genetic material (which can be either DNA or RNA). In some instances (such as herpes simplex), the virus can also contain an lipid envelope that serves as a membrane.
Example Question #2 : Understanding Animal And Plant Viruses
Which of the following best describes retroviral DNA that has been incorporated into a host genome?
Provirus
Endogenous retrovirus
Virion
Retroelements
Exogenous retrovirus
Provirus
The correct answer is provirus. A retrovirus uses its own reverse transcriptase to synthesize DNA from its RNA. This DNA is then incorporated into the host genome via the integrase enzyme. The viral DNA in the host genome is referred to as a provirus; the host cell transcribes and translates the provirus to produce new copies of the virus, along with its own genome.
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