All High School Biology Resources
Example Questions
Example Question #1 : Transcription
Which of the following proteins is responsible for transcribing messenger RNA?
RNA polymerase II
RNA polymerase I
RNA polymerase III
DNA polymerase
RNA polymerase II
RNA polymerase II is the primary protein responsible for generating mRNA.
RNA polymerases I and III transcribe other RNAs (such as tRNA and rRNA). DNA polymerase is responsible for DNA replication during the S phase of the cell cycle.
Example Question #1 : Understanding Transcription Proteins
Transcription factors bind to __________, after which RNA polymerase can bind to these transcription factors in order to open the DNA double helix.
a ribosome
promoter sites
a gene
a peptide strand
the 3' side of the DNA strand
promoter sites
During transcription, transcription factors will bind to promoter sites on the 5' side of the gene to be transcribed. Although the answer "a gene" is technically correct, the more accurate answer is promoter site—the region of DNA that initiates transcription.
A peptide strand is the product of translation, and does not bind transcription factors.
Ribosomes help read the RNA that is eventually transcribed from the DNA, but transcription factors do not interact directly with the ribosomes.
Example Question #1 : Rna
Where in a plant cell is mRNA synthesized?
The nucleolus
The nucleus
The ribosome
The Golgi apparatus
The cytoplasm
The nucleus
Plant cells are eukaryotic, thus they have nuclei. The process of mRNA synthesis is called transcription. Transcription occurs in the nucleus in eukaryotes. In prokaryotes, it occurs in the cytoplasm. Note that rRNA synthesis and ribosome assembly takes place in the nucleolus.
Example Question #2 : Rna
What would most likely result from the insertion of a single nucleotide during transcription?
None of these
A frameshift mutation
A truncated mRNA molecule
A truncated protein
No mRNA will be produced
A frameshift mutation
The genetic code is read in triplets. The addition of a single nucleotide would shift the triplets such that they are no longer read in the correct frame. This is called a frameshift mutation. A truncated protein could be a result of an insertion, but this is not the most likely result. Early transcriptional termination would not be a likely result of an insertion, since this is mediated by long GC repeats or a protein called rho in prokaryotes. Eukaryotic transcriptional termination is not well-understood.
Example Question #1 : Rna
Which of the following describes the process by which RNA polymerase II binds to the promoter of a target gene and recruits transcription machinery?
Splicing
Elongation
Initiation
Termination
Initiation
The first stage of transcription is initiation, in which RNA polymerase II (PolII) engages the promoter and recruits the general transcription machinery. Following initiation, PolII travels down the length of the gene, producing a transcript (elongation). Finally, transcription is terminated, and PolII is removed from the gene. Following transcription, immature heterogeneous RNA (htRNA) can be processed during splicing to become mature messenger RNA (mRNA).
Summary of steps:
Initiation, elongation, termination, splicing
Example Question #3 : Transcription
An mRNA strand is transcribed from the following template strand on DNA.
5'-CGAATGGCAT-3'
What is the mRNA strand created from this template strand?
5'-ATGCCATTCG-3'
5'-GCUUACCGUA-3'
5'-AUGCCAUUCG-3'
5'-GCTTACCGTA-3'
5'-AUGCCAUUCG-3'
When transcribing from a template strand, the new strand is synthesized in the opposite direction, much like in DNA replication. This will result in antiparallel strands. Also, we need to replace thymine with uracil, because RNA uses uracil in place of thymine.
Template: 5'-CGAATGGCAT-3'
Answer: 5'-AUGCCAUUCG-3'
To see these pairs match up, the 3' end of the answer must align with the 5' end of the template.
Template: 5'-CGAATGGCAT-3'
Answer (3'-5'): 3'-GCUUACCGUA-5'
Example Question #4 : Transcription
What is the function of transfer RNA (tRNA)?
To bind to specific amino acids and facilitate peptide bond formation
To transfer genetic information from the nucleus to the cytosol of a eukaryotic cell
To bind with proteins and fold into a globular form to make up the ribosome structure
To convert the deoxyribose sugar on DNA to ribose to be incorporated into RNA
To bind to specific amino acids and facilitate peptide bond formation
There are several types of RNA, but four main types: messenger RNA (mRNA), transfer RNA (tRNA), ribosomal RNA (rRNA), and heteronuclear RNA (htRNA).
Heteronuclear RNA is the direct product of transcription, prior to post-transcriptional modification. htRNA is unable to exit the nucleus until it has undergone RNA splicing to remove introns, addition of the poly-A tail, and addition of the 5' cap. At this point, the htRNA has matured to become functional mRNA.
Messenger RNA is the final transcription product from DNA and used as the template for protein translation. It carries genetic information in the form of codons from the nucleus to the cytosol to create protein chains.
Transfer RNA binds to specific amino acids and helps add them to protein chains during translation. tRNA molecules enter active sites in the ribosome and match an anticodon region to the mRNA template codon before transferring their amino acid cargo to the polypeptide chain.
Ribosomal RNA associates with proteins and is used to form the structure of the ribosomes.
Example Question #4 : Rna
Which of the following processes creates messenger RNA from a DNA?
Replication
Respiration
Translation
Transcription
Transcription
The process of forming messenger RNA from a strand of DNA is called transcription. Replication is the creation of new DNA from the original DNA strands. Translation is the creation of a protein chain from the messenger RNA strand using transfer RNA.
Example Question #1 : Understanding Transcription Regulation
All of the following statements about RNA are true EXCEPT __________.
RNA and DNA both have a sugar-phosphate backbone in their molecular structure
adenine always pairs with thymine and cytosine always pairs with guanine in RNA
adenine always pairs with uracil and cytosine always pairs with guanine in RNA
RNA is most frequently a single-stranded molecule
RNA contains the carbohydrate ribose
adenine always pairs with thymine and cytosine always pairs with guanine in RNA
It is important to remember the base-pairing rules when discussing both DNA and RNA because they are the rules by which all of transcription and translation occur. In RNA, uracil takes the place of thymine, creating an A-D pair instead of an A-T pair. The structure of RNA is a single strand of alternating ribose and phosphate groups with nitrogenous bases attached to the ribose. One way that DNA and RNA differ is that DNA contains deoxyribose sugar while RNA contains the ribose sugar.
Example Question #2 : Understanding Transcription Regulation
Protein synthesis is the process by which proteins are made in cells. The proper order and location of the two main parts of protein synthesis are __________.
1) Translation at the ribosome 2) Transcription in the nucleus
1) Transcription at the ribosome 2)Translation in the nucleus
1) Transcription in the nucleus 2) Translation at the ribosome
1) Translation in the nucleus 2) Transcription in the endoplasmic reticulum
1) Transcription in the cytoplasm 2) Translation in the nucleus
1) Transcription in the nucleus 2) Translation at the ribosome
Transcription must occur first because it is the process that copies the genetic code from the DNA, and it must occur in the nucleus because DNA is too large a molecule to leave the nucleus. Next comes translation, which is the reading of the "photocopied" code (mRNA) after it leaves the nucleus and connects with a ribosome. After this, the mRNA binds with ribosomes and is translated to create proteins.
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