Biochemistry : Michaelis-Menten Graphs
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Example Question #1 : Michaelis Menten Graphs
In a Lineweaver-Burk plot, the slope is __________.
A Lineweaver-Burk is a double-reciprocal of the Michaelis-Menten equation. The equation for the graph, in 
From this graph, we can see that the slope is 
Example Question #1 : Michaelis Menten Graphs
In a Michaelis-Menten plot of enzyme kinetics, the reaction rate is plotted as a function of substrate concentration. Why is it that as substrate concentration increases, the curve of the graph levels off and reaches a plateau?
All of the substrate has been converted into product
This only happens when a competitive inhibitor is present
This only happens when a non-competitive inhibitor is present
The enzyme becomes degraded
The enzyme becomes saturated
The enzyme becomes saturated
In a classic Michaelis-Menten graph, the y-axis represents reaction rate and the x-axis represents substrate concentration. At low substrate concentrations, the reaction rate increases sharply. But as the substrate concentration climbs, the reaction rate begins to increase less and less until it comes to a point where it plateaus into a flat line.
The reason this happens is because the enzyme becomes saturated with substrate. When substrate concentration is low, many of the enzymes in solution aren't doing anything, so they're readily available to convert substrate into product. Thus, adding even just a little bit of substrate will result in a dramatically increased reaction rate. When a high concentration of substrate is present, all of the enzymes in solution are busy. In other words, as soon as an enzyme converts a substrate into product, it immediately becomes occupied with another substrate. Since this process can't occur any faster unless more enzymes are added, all of the other substrate in solution have to wait their turn. Thus, any increase in substrate concentration under these circumstances results in very little, if any, increase in reaction rate.
Example Question #1 : Michaelis Menten Graphs
Where on a Michaelis-Menten graph is the slope of reaction velocity linear?
When enzyme concentration is low
When substrate concentration is high
When enzyme concentration is high
It is never linear
When substrate concentration is low
When substrate concentration is low
When substrate concentration is high, all of the active sites can be constantly occupied by enzyme. Further, the active sites can be saturated by enzyme when concentration is very high. But, when the concentration of substrate is low, the reaction velocity is considered to be linear.
Example Question #71 : Enzyme Kinetics And Inhibition
On a Lineweaver-Burk plot, which of the following is correct?
X intercept =
Slope =
Y-intercept =
X-axis is
Slope =
Slope =
On a Lineweaver-Burk plot, the following are the correct matches regarding points of importance?
X-axis =
Y-axis =
X-intercept =
Y-intercept =
Slope =
Example Question #1 : Michaelis Menten Graphs
In the presence of a competitive inhibitor, which of the following changes will always be observed on the Lineweaver-Burk plot?
The lines will always be exactly the same
The y-intercept for the uninhibited line will be higher than that for the inhibited line
The x-intercept will be closer to the origin for the uninhibited line that for that of the inhibited line
The y-intercept will be the same for the inhibited and uninhibited line
The x-intercept of the inhibited line will be the same for both lines
The y-intercept will be the same for the inhibited and uninhibited line
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